Composite
Part:BBa_K1487076:Design
Designed by: Samantha Bermudez Group: iGEM14_UT-Dallas (2014-10-06)
eYFP Reporter for tcpI gRNA target with strong promoter.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.
Source
Part was assembled using complementary primers to match a 20 base pair sequence in the tcpI coding region.